Uracil-DNA Glycosylase (UDG)

Detection enzymes and reagents

Product Overview

Uracil-DNA Glycosylase (UDG)

Recognizes and removes uracil from DNA. In compatible dUTP-based PCR workflows, UDG can reduce carryover contamination from previous amplicons.

Reference price: ¥580 / 100μL (indicative)

Quantity

Technical Overview

Uracil-DNA glycosylase (UDG, also called UNG) recognizes uracil bases in DNA and cleaves the N-glycosidic bond, leaving an abasic site. It is a starting enzyme in base-excision repair.

When used with a compatible dUTP-containing PCR workflow, UDG can treat residual products from earlier amplifications and reduce carryover-related false positives. Compatibility with the specific reaction mix and thermal program should be validated.

Specifications

  • Specific activity: 1.3 × 10⁵ U/mg
  • Expression yield: 30 mg/L
  • Pack size: 100 μL
  • Reference reaction: 25°C for 15 min

Applications

  • PCR carryover-contamination control
  • Molecular cloning
  • Seamless DNA assembly
  • Early cancer-detection research

Product Features

  • High specific activity
  • Stable expression
  • High specificity
  • Good stability
  • Reliable results

Source and Storage

Storage
Store at −20°C and avoid repeated freeze–thaw cycles.

Technical Inquiry

For batch-specific specifications, storage conditions, suitability and application guidance, contact our team with your target substrate and use case.

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