Fructanase for Food Bioprocessing

Application Solution

Overview

Assess fructan substrate conversion through enzyme selection, bench-scale trials and process optimization.

How should an enzyme and operating conditions be selected for fructan processing?

Challenges

  • Fructans from different sources vary in composition and polymerization
  • Temperature, pH and reaction time affect conversion
  • Laboratory metrics must connect to actual production conditions

Recommended Approach

  1. 1. Characterize the raw material

    Determine fructan source, concentration, solubility and desired conversion products.

  2. 2. Run a range of bench trials

    Test enzyme dose, temperature, pH and time in a structured range to identify a workable window.

  3. 3. Validate scale-up

    Assess mass transfer, equipment and final-product specifications under scaled conditions.

Potential Benefits

  • High expression level
  • High enzyme activity
  • Applicable to food and bioprocessing
  • Supports process-condition optimization

Related Products

Fructanase

A GH32-family enzyme that hydrolyzes β-2,6-fructofuranosidic bonds in fructans, producing fructooligosaccharides and some fructose.

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Questions

What information is needed to determine dosage?

Provide raw-material source, substrate concentration, target conversion, reaction temperature, pH and allowable reaction time.

Can laboratory results be used directly for production scale-up?

Not without validation. Laboratory and plant equipment differ; run pilot or equipment-relevant scale-up tests before use.

Discuss Your Application

Implementation conditions should be validated for your materials and process.

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